Development and characterization of microsatellite markers for the medicinal plant Smilax brasiliensis (Smilacaceae) and related species1
نویسندگان
چکیده
UNLABELLED PREMISE OF THE STUDY A new set of microsatellite or simple sequence repeat (SSR) markers were developed for Smilax brasiliensis, which is popularly known as sarsaparilla and used in folk medicine as a tonic, antirheumatic, and antisyphilitic. Smilax brasiliensis is sold in Brazilian pharmacies, and its origin and effectiveness are not subject to quality control. • METHODS AND RESULTS Using a protocol for genomic library enrichment, primer pairs were developed for 26 microsatellite loci and validated in 17 accessions of S. brasiliensis. Thirteen loci were polymorphic and four were monomorphic. The primers successfully amplified alleles in the congeners S. campestris, S. cissoides, S. fluminensis, S. goyazana, S. polyantha, S. quinquenervia, S. rufescens, S. subsessiliflora, and S. syphilitica. • CONCLUSIONS The new SSR markers described herein are informative tools for genetic diversity and gene flow studies in S. brasiliensis and several congeners.
منابع مشابه
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عنوان ژورنال:
دوره 1 شماره
صفحات -
تاریخ انتشار 2013